In the clinical environment, measurements of some characteristics of the skeletal muscle are currently used to assess the severity of a neuromuscular disease or in some cases to assist in making a diagnosis. But a quantitative method of evaluation has not yet been introduced satisfactorily. In this paper, the skeletal EMG(biceps muscle, masseter muscle) analysis has been processed both in the time and in the frequency domain by designing the digital signal processing system based on pentium PC and transputer (IMS 7805). The experiment have been performed in five normal subjects, and various parameters have been statistically tested and compare4 As a results, the effective parameters obtained for the evaluation of skeletal EMG electrical activity are turn analysis, MiTi, MiTa, IEMG, PDF in the time domain, and are mean frequency, median frequency, skewness, kurtosis, muscle fatigue slope in the frequency domain. The designed H/W and S/W in this study can be used effectively for the establishment of EMG data base and for clinical research.