It was carried out to find the carbohydrate metabolism for the glycogen utilization and the effects of low temperature(1$circ$C, 5$circ$C)on embryonic development of the silkworm, Bombyx mori L. The content of sorbitol and glycerol in the chillihg eggs stored at 5$circ$C or 1$circ$C on 24 hours after oviposition showed the highest level on the 50th day. After that, the corresponding value in the eggs stored at 5$circ$C were decreased, and the value in the eggs stored at 1$circ$C maintained the similar level. The trehalose level was rapidly increased on 50$sim$60th day or thereabouts, and then, it was accumulated. When the eggs stored at low temperature were tranaferred and developed the embryo at 25$circ$C, sorbitol and glycerol were respectively changed to glycogen. Futhemore, the content of trehalose and glucose showed specific of patterns with each embryonic stage at low temperature. With the aforementioned results, the intemediary metabolism of carbohydrate was discussed with regard to embryonic development of non-diapause eggs of silkworms.