- 균형 생육조건하에서 Actinobacillus sp. EL-9에 의한 Poly-$eta$-Hydroxybutyrate의 생산
- ㆍ 저자명
- 손홍주,이상준
- ㆍ 간행물명
- 산업미생물학회지
- ㆍ 권/호정보
- 1996년|24권 3호|pp.344-351 (8 pages)
- ㆍ 발행정보
- 한국미생물생명공학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Microorganisms growing alcoholic distillery wastes were isolated from soil. Of them, the selected strain EL-9 had a capability of accumulating poly-$eta$-hydroxybutyrate (PHB) when grown in alcoholic distillery wastes. The strain EL-9 was identified as a genus Actinobacillus based on the morphological, cultural, and biochemical characteristics. The strain EL-9 was named temporarily as Actino- bacillus sp. EL-9. The optimal temperature and pH for cell growth of Actinobacillus sp. EL-9 were 30$circ$C and 7.0, respectively. The optimal medium compositions for cell growth comprised glucose 2%, NH$_{4}$NO$_{3}$ 0.15%, Na$_{2}$HP0$_{4}$-12H$_{2}$O 0.25%, KH$_{2}$PO$_{4}$ 0.25%, MgSO$_{4}$4-7H$_{2}$O 0.15%, FeSO$_{4}$-7H$_{2}$O 0.005%, CaCl$_{2}$-2H$_{2}$O 0.003% and trace element solution 5m/l. To investigate the optimal conditions for PHB production, two-stage culture technique was used. The result showed that the optimal conditions for PHB production are identical those for cell growth. When propionic acid was added as a cosubstrate, PHB/HV copolymer was produced.