The stress and vibration analysis of stiffened box structure is investigated by experiment and FEM analysis. The effects of stiffener number and box section shape on the structure response are presneted. The 1st natural frequency of stiffened folded byx structure with a stiffeners is 300 Hz. It is highter than that of stiffened rectangular box structure with 5 stiffeners, which is 251 Hz. Maximum deflection of folded box structure with thickness of 1 mm is lower than that of rectangular box structure with thickness of 1 or 2 mm. The natural frequencies of box structures are increeased with the number of stiffener, while the deflections are decreased with the number of box structures. When we compare between fundamental frequency (251 Hz) of stiffened (with 5 stiffeners) and one (137.64 Hz) of unstiffened rectangular box structure under clamped-clamped boundary condition, the ratio of frequency increase is 82%. The stiffened structures of 2 mm thickness can reduced to 120% of maximum deflection of 1 mm thickness rectangular box structures.