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돼지 미성숙 난포란의 유리화 동결융해후 FDA 처리가 체외수정과 배 발육에 미치는 영향 II. 난구세포의 부착정도가 돼지난포란의 체외성숙에 미치는 영향
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  • 돼지 미성숙 난포란의 유리화 동결융해후 FDA 처리가 체외수정과 배 발육에 미치는 영향 II. 난구세포의 부착정도가 돼지난포란의 체외성숙에 미치는 영향
  • Effects of FDA Treatment after Vitrified Freezing on In Vitro Fertilization and Development of Porcine Follicular Oocytes II. Effect of Degree of Cumulus Cell Attachment on In Vitro Maturation of Porcine Follicular Oocytes
저자명
김영훈,김중계
간행물명
韓國受精卵移植學會誌
권/호정보
1996년|11권 3호|pp.233-240 (8 pages)
발행정보
한국수정란이식학회
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정기간행물|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

This study was carried out to determine the effect of cumulus cell attachment and various factors on in vitro maturation of pig foflicular oocytes. Oocytes with various configuration of cumulus cell mass were collected ftom ovaries of mature gilts by asperating with syringe equipped with needles of different gauges, follicle size and with or without cumulus cells. They were cultured in TCM-199 mediun containing FGS(fetal calf serum) for 30~48 hours in incubator with air containing 5% $CO_2$ at 38.5$^{circ}C$. Mter orcein staining at in vitro maturation condition, GV, GVBD, anaphase, telophase and M II were observed. Results are surumarized as follows: 1. Recovery rates were 55.8, 55.5 and 34.4% when the cumulus-compacted oocytes were collected with 18, 21, 26 gauge needles of syringes, respectively. 2. 79% of oocytes with compacted cumulus cells were at GV stage and most of the oocytes with partially denuded and denuded cumulus cells were from GVBD to M- II stages. 3. Percentage of mature oocytes among those which are follicular diameter of 1~2, 3~6 and over 6 mm was 42.6, 53.2 and 60.8%, respectively. 4. Percentage of mature oocytes among those which are compacted, partially denuded and denuded was 60.5, 46.2 and 35.4% respectively. 5. Percentage of mature oocytes in co-cultured with monolayers of cumulus cells was higher (57.1%) than that found with oocytes cultured alone (53.4%).