To predict the service life of an organic vapor respirator cartridge, the breakthrough curve of respirator was simulated using a fixed-bed adsorption model and compared with that of sampling tube. And the effects of bed porosity, length to diameter ratio and flow rate of the sampling tube were studied. The life time of respirator cartridge was increased with the decrease of particle size and bed porosity. And the breakthrough time of sampling tube was affected by the flow rate, however not by the length to diameter ratio. The 10% breakthrough time of the sampling tube was corresponded with that of cartridge.