- RAPD(Random Amplified Polymorphic DNA)법을 이용한 한약재의 판별 연구
- ㆍ 저자명
- 김대원,김도균,안선경,조동욱,Kim. Dae-Weon,Kim. Do-Kyun,An. Sun-Kyong,Cho. Dong-Wuk
- ㆍ 간행물명
- 韓國韓醫學硏究院論文集
- ㆍ 권/호정보
- 1997년|3권 1호|pp.153-167 (15 pages)
- ㆍ 발행정보
- 한국한의학연구원
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Conventionally, identification and classification methods of natural products include the morphological survey and assay of chemical disposition, sing these methods, however, is not satisfying for the precise identification of natural products because they are often valiable in the compositions and morphology To standardize the natural products identification and classification, genomic DNA analysis such as RAPD, RFLP and Amp-FLP can be adopted for this purpose. In this study, various ginsengs and bear gall bladder were tested for the development of genetic identification and classification method. Varieties of ginsengs such as, P. ginseng, P. quinquefolium, P. japonicus and P. notoginseng, were genetically analyzed by RAPD. Also, DNA isolated from Bear blood and gall bladder, Ursus thibetanus, Ursus americanus and Ursus arctos, were analyzed by the same method. The results demonstrated that the identification and classification of bear gall bladder and various ginsengs were possible by RAPD analysis. Therefore, this method was thought to be used as a additional method for the identification and classification of other natural products.