Cellulose를 효과적으로 분해하는 효소를 생산하는 미생물인 strain No. 33 균주를 토양으로부터 분리하였다. 본 균주의 배양상등액을 2.0%의 CMC가 함유된 agar 평판배지에 paper disc법을 이용하여 loading 후 cellulose 분해활성을 검토하였을 때, disc 주위로 노란색의 환이 형성되는 것을 확인할 수 있었다. 본 균주를 동정하기 위하여 전자현미경으로 그 형태를 관찰한 결과 본 균주는 간균의 형태였으며, 생리학적 특성을 검토한 결과 Bacillus속으로 동정되었다. 이에 본 균주를 Bacillus sp. No. 33으로 명명하였다. 본 균주는 1.0% glucose, 3.0% yeast extract, 0.5% $KH_2PO_4$, 0.02% $MgSO_4{cdot}7H_2O$가 함유된 pH 7.0의 배지에서 $30^{circ}C$로 39시간 동안 진탕배양하였을 때 최대 균체생육을 나타내었다. 효소생산은 4.0% CMC, 2.0% yeast extract, 0.5% $KH_2PO_4$, 0.04% $MgSO_4{cdot}7H_2O$, pH 7.0의 조건에서 $30^{circ}C$, 42시간 동안 진탕배양하였을 때 최대 효소생산을 나타내었다.
The strain No. 33, which produces cellulose-degrading enzyme, was isolated from soil. Yellow halo was identified when the culture supernatant of the strain was loaded onto agar plate containing 2.0% CMC using paper disc method. From scanning electron microscopic observation, the morphology of the stain was rod-shaped. For identification, several biochemical characteristics were tested, and this strain was identified to Bacillus sp. So, we named this strain as Bacillus sp. No. 33. The maximal growth was observed when the stain was cultured in the medium containing 1.0% glucose, 3.0% yeast extract, 0.5% $KH_2PO_4$, 0.02% $MgSO_4{cdot}7H_2O$, pH 7.0 at $30^{circ}C$ for 39 hours with shaking. The maximal enzyme production was accomplished using the medium containing 4.0% CMC, 2.0% yeast extract, 0.5% $KH_2PO_4$, 0.04% $MgSO_4{cdot}7H_2O$, pH 7.0 at $30^{circ}C$ for 42 hours with shaking.
