고추냉이의 배수체를 유도하기 위하여 EMS와 colchicine을 처리하였을 때 변화되는 단백질과 과산화효소 활성은 다음과 같이 나타났다. 발아율은 무주산이 울릉도산 보다 높았으며, GA3 100 ppm+ EMS 10 ppm 혼용처리시 무처리구에 비하여 모두 6배 이상 증가되었다. Mutagen 처리후 생존율도 울릉도산 보다 무주산에서 더 높게 나타났으며, 과산화효소 활성과 와사비의 초장은 감소되어 나타난 반면, 줄기와 뿌리는 비대되는 경향을 보였다. 무주산과 울릉도산의 단백질 양상이 비슷하고, mutagen을 처리했을 때 polypeptide spot수가 증가되었으나 단백질 pattern은 다르게 나타났고, 특히 EMS 처리는 무처리구에 비하여 현저하게 다른 양상을 보였다.
This study was investigated on the effects of colchicine and EMS. induced mutation, plant on the germination and polypeptide pattern and peroxidase activity monitored by two dimensional gel analysis in Wasabia japonica Matsum (wasabi). Germination rate of Muju was higher than that of Ulrung-Do and optimum concentration for germination was appeared 100 ppm GA3 containing with 10 ppm BAP in these cultivars. Survived plants rate of Muju was higher than that of Ulrung-Do after colchicine and EMS treatment. Peroxidase activity and plant height were decreased by mutagen treatments, while incresed in root and stem thickness. The number of protein spots and pattern showed difference between Muju and Ulrung-Do . The plants treated mutagen increased polypeptide spots, especially EMS treatment showed more different polypeptide pattern compared to control.
