Aspergillus awamori(A)와 Zymomonas mobilis(Z)로 구성된 혼합고정화 배양계(A-Z 36계)의 특성을 이용하여 생전분으로 부터 직접 에탄올생산을 행할 때 투입되는 에너지를 절약할 목적으로 비가열살균 배양법을 검토하였다. A-Z 36계에 의한 비가열살균 배양은 배지에 함유된 세균 때문에 어려웠다. Sorbic acid, benzoic acid, dehydroacetic acid, p-hydroxybenzoic acid, Vantocil IB, Neupectin-L 등의 여러 가지 살균제 중에서 Vantocil IB와 Neupectin-L은 잡균의 오염없이 대조구와 비슷한 속도로 기질을 분해하였다. 0.1% Neupectin-L은 대조구보다 약간 높은 6.9 g/l(대조구 : 6.4 g/l)의 에탄올을 생산하였다$(Y_{p/s}:0.39)$. 0.1% Neupectin-L을 첨가한 유가배양에서는 2%기질을 기본으로 5회분의 기질을 첨가하여 34 g/l 에탄올을 얻어 2.0 g/l/day의 생산성을 나타내었다.
In order to reduce energy input in direct ethanol production from raw starch by co-immobilized Aspergillus awamori(A) and Zymomonas mobilis(Z), A-Z 36 culture system which was changed to anaerobic after 36 h of aerobic fermentation without sterilization was investigated. This immobilized cell system can not be carried out under unsterile conditions because of growth of microbial contaminants from original medium. Among some food additives such as sorbic acid, benzoic acid, dehydroacetic acid, p-hydroxybenzoic acid, Vantocil IB and Neupectin-L, Vantocil IB and Neupectin-L were a potent antibacterial agent in A-Z 36 culture cell system and were not affected in hydrolysis of substrate as compared with the case of control. Ethanol yield(6.9 g/l) in system of addition of 0.1% Neupectin-L was slightly higher than that in control(6.4 g/l). When 2% starch was fed five times in fed-batch culture with 0.1% Neupectin-L, ethanol yield and productivity were 34 g/l and 2.0 g/l/day, respectively.
