In order to investigate the effect of Daewhajungeum on the isolated organs, gastrointestinal tract and C.N.S., some kinds of experiments using mice and rats were done. The results were as follows; 1. Daewhajungeum showed the inhibitory effect on the smooth muscle contraction of the isolated ileum and colon induced by acethylchorine chloride and barium chloride in mice. 2. Daewhajungeum showed inhibitory effect on the contraction of the fundus-strip induced by acethylchorine chloride and barium chloride in rats. 3. The preventive effect on pyloric ulcer, indomethacin induced ulcer and ethanol-HCl induced ulcer of mice was recognized. 4. The effect on the acidity of gastric juice was suppressed, but the effect on the pepsin output was not recognized. 5. The transport ability of small and large intestine was significantly decressed. 6. The analgesic effect and the sedative effect were recognized so that the effect of the C.N.S was suggested.