The purpose of this study was to determine the inheritance of chlorosis under low temperature at seedling stage of rice. The expression of chlorosis among 21 rice cultivars treated at $18^{circ}C$ showed in one Tongil type cultivar, Milyang 23, and two Indica type cultivars, IR 36 and Dular. The sign of chlorosis was not appeared in all of the japonica type cultivars under the same treatment. The reduction of chlorophyll content under the low temperature was differed from varietal group and cultivars. The $F_2$ population from a cross between Toyohatamochi and Dular segregated 3:1 ratio for normal and white phenotypes of third leaf. From the result, it was suggested that the chlorosis was controlled by a single recessive gene. The chlorosis rice detected from this study will be used as a good material for the genetic study related to photosynthetic ability of rice.