- implementation and its limitations
- implementation and its limitations
- ㆍ 저자명
- Nahm. Kie-B.,Shin. Eun-S.,Ryoo. Seok-M.
- ㆍ 간행물명
- Journal of the Optical Society of Korea
- ㆍ 권/호정보
- 1997년|1권 2호|pp.90-93 (4 pages)
- ㆍ 발행정보
- 한국광학회
- ㆍ 파일정보
- 정기간행물|ENG| PDF텍스트
- ㆍ 주제분야
- 기타
The shallow depth of focus in conventional light microscopy hinders the observation of the whole image when the object is thicker than the depth of field. Most of the existing techniques measured the object distance, which is not necessarily the actual distance of each pixel in the image. We implemented a means of determining the "best focus" of each pixel and located the height of object points by sectioning at different sample heights. Combining the height information and its gray values together, we obtained an image where the blur from the finite depth of focus is eliminated. Limitations of the technique are discussed together with composed images.ed images.