The adhesive properties and fatigue resistance of polyester cord in rubber matrix were investigated in terms of coating conditions and types of adhesive agents. The polyester cord was coated with diisocyanate adhesive agent and then resorcinol-formaldehyde latex(RFL). Two types of diisocyanate adhesive agent were used. One is made from hexamethylene diisocyanate (aliphatic type), and the other is made from m-xylene diisocyanate (aromatic type). The adhesive properties were measured by the Pull-Out method, and evaluated with regard to the energy of adhesion and the maximum load. The fatigue resistance was checked by disc fatigue tester. The adhesive properties of polyeser to rubber matrix were affected by the types of diisocyanate adhesive agent used. Namely, the energy of adhesion was higher in aliphatic type and the maximum load was higher in aromatic type. The fatigue resistance was also affected by the adhesive properties, especially the energy of adhesion rather than the maximum load. We concluded that while the energy of adhesion was kept high, the repeated stress and strain were distributed well all over the fiber resulting the good fatigue resistance.