The experiments were conducted to improve the mass production of Nile tilapia, Oreochromis niloticus, seed in closed recirculation systems. It was observed that smaller females (340g av. body weight) under lower stocking density (1.2 kg/$m^2$)) group produced 3.6 times more fry than larger females (612g av. body weight) under higher stocking density (2.1kg/$m^2$) group when the fry were collected at 30 days after the broods stocked. The clutch removal method where the eggs and sacfry were collected from female mouth followed by artificial incubation showed improved seed production, when compared to the traditional natural mouth-brooding method by harvesting free swimming fry from brood tanks (P<0.05). Under the clutch removal method, short-term incubatin of brooders (14 days) was proven to be more effective than long-term incubatin (21 days). Hatching success of clutch removed eggs was ranged from 55.7 to 91.5% in 1.8$ell$ of upwelling incubators depending on the different development stages.