The purpose of this study was to investigate the potential hypoglycemic effect of adlay diets when total fiber consumption was controlled in streptozotocin-induced diabetic rats. Forty eight rats were fed for 3 weeks with either controlled in streptozotocin -induced diabetic rats. Forty eight rats were fed for 3 weeks with either control diets or experimental diets : raw mille adlay (RMA) raw whole adlay(RWA) , steamed milled adlay(SMA) or roasted mille adlay(OMA). The composition of the AIN-76 diet was modified to ensure the same composition of protein, carbohydrate , rat, and fiber between the control diet and experimental diets. The concentrations of glucose , insulin, glycogen, and protein in plasma, liver, or skeletal muscle were compared . Compared to diabetic control rats, plasma postprandial glucose levels tended to be decreased in RMA, RWA, SMA and OMA rats until the 2nd week, but no difference was shown at the 3 rd week. There was no significant difference in insulin levels among those groups. After glucose loading, the plasma glucose level of SMA was lower than that of diabetic control rats throughout 2 hrs. Liver glycogen was lower than control values in RMA and RWA rats and not different in SMA and OMA rats. The muscle protein level of RMA, RWA, SMA, and OMA rats tended to be lower than in diabetic control rats. There was no significant difference in muscle glycogen among groups. These results suggest that the hypoglycemic effect of an adlay diet is not significant when the amount of total fiber consumption is controlled.