This study was conducted to investigate the effect of levels of nitrogen fertilizer on the nitrate accumulation in Italian ryegrass. Italian ryegrass was grown at the experimental field, College of Agriculture, Chonnam National University, Kwangju from September, 29, 1987 to June, 24, 1988. The experiment was arranged as a randomized complete block design with three treatment(2.5, 7.5 and 15kg-NIlOa). The results obtained are summarized as follow; Nitrate nitrogen content of the stem, leaves and whole was increased as increasing nitrogen fertilizer(P< 0.05). Total nitrogen content in the leaves was much higher than that of stem, and not significance among the levels of nitrogen fertilizer. Total water soluble carbohydrate content in stem was higher than that in the leaves and decreased after cutting as increasing nitrogen fertilizer(P < 0.05)