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Biomass Production of Saccharomyces cerevisiae KFCC 10823 and Its Use in Preparation of Doenjang
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  • Biomass Production of Saccharomyces cerevisiae KFCC 10823 and Its Use in Preparation of Doenjang
  • Biomass Production of Saccharomyces cerevisiae KFCC 10823 and Its Use in Preparation of Doenjang
저자명
Yoo. Jin-Young,Kim. Hyeon-Gyu,Kwon. Dong-Jin
간행물명
Journal of microbiology and biotechnology
권/호정보
1997년|7권 1호|pp.75-80 (6 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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An ethanolic fermentation process was developed for preparing Doenjang with high ethanol. Higher and efficient viable cell production of salt-tolerant ethanolic yeast is a prerequisite for the successful commercial-scale process of ethanol production during Doenjang fermentation. Culture conditions of salt-tolerant yeast, S. cerevisiae KFCC 10823, was studied in terms of the effect of several environmental and nutritional factors. Viable cell numbers were the highest in a medium containing the following components per liter of water: soysauce, 300ml; dextrose, 50 g; beef extract, 5 g; yeast extract, 5 g; $KH_2PO_4$, 5 g; NaCl, 50 g. The optimal culture conditions of S. cerevisiae KFCC 10823 were pH 5.5, $25^{circ}C$, 200 rpm and 0.5 vvm. Yeast viability during batch fermentation was gradually decreased to a level less than $90{\%}$ after 35 hours. The maximum cell number was $2.2{ imes}10_7$ cells/ml at the optimal condition. Doenjang prepared with ethanolic yeast was ripened after 45 days at $30^{circ}C$. This Doenjang contains 470 mg% of amino nitrogen and 2.5% ethanol. The shelf-life at $30^{circ}C$ was theoretically estimated as 444 days.