Bio-diesel oil is a great possibility to solve the pollution problem caused by the exhaust gas from diesel engine vehicles. Recently the use of bio-oils in disel engines has received considerable attention to the forseeable depletion of world oil supplies. So, Bio-diesel oil has been attracted with attentions as an alternative and clean energy source. The objective of this paper is to experimentally investigate the characteristic of performance using light oil, rice-bran oil, heated rice-bran oil, rice-bran oil treated with ultrasonic energy. We included rice-bran oil and applied ultrasonic energy to highly viscous bio-oils. These methods seems to have never been tried yet. The final data may be able to be applicated for the design of the diesel engine using an alternative fuel.