Comparative osteology of Chromis notata, C. analis and C. fumea was studied based on the samples collected from the Korean waters. The three species are distinguished by the shape of cranium, visceral skeleton, vertebra and caudal skeleton. Chromis notata has a glossohyal which is slightly curved in shape while two other species are deeply curved. C. analis has three spiniform procurrent caudal rays while others have two. C. fumes could be distinguished easily by the shape of preopercle which is serrate in posterior margin. This investigator reveals following bones as new taxonomic criteria for the genus Chromis in Korea. In cranium, height of supraoccipital, height of process of parasphenoid and anterior margin of basisphenoid, in visceral skeleton, presence of fine papilla-like process at the surface of preopercular sensory canal, in orbital bone, width of preorbital, in hyoid arch, shape of glossohyal, union state between lower hypohyal and ceratohyal and union state between ceratohyal and epihyal, in shoulder girdle bone, dorsal margin of posttemporal, width of upper postclavicle, posterior end of lower postclavicle and posterior margin of first actinost bone, in vertebrae, beginning point of parapophysis, in caudal skeleton, upper shape of second neural spine of pleural centrum.