Two new triterpenoid saponins, named segetoside D and E, have been isolated from the seeds of Vaccaria segetalis. On the basis of chemical reactions and spectral data, structures of segetoside D and E have been established as: $28-O-[{eta}-D-xylopyranosyl-(1{ ightarrow}4)-{alpha}-L-rhamnopyranosyl-(1{ ightarrow}2)]-[5-O-acetyl-{alpha}-arabinofuranosyl(1{ ightarrow}3)]-[4-O-acetyl-{eta}-D-fucopyranosyl]-quillaic;acid-3-O-[{eta}-D-galactopyranosyl(1{ ightarrow}2)]6-O-methyl;ester-{eta}-D-glucuronopyranoside$ and $28-O-[{eta}-D-xylopyranosyl-(1{ ightarrow}4)-{alpha}-L-rhamnopyranosyl-(1{ ightarrow}2)]-[5-O-acetyl-{alpha}-arabinofuranosyl(1{ ightarrow}3)]-[4-O-acetyl-{eta}-D-fucopyranosyl]-quillaic;acid;-3-O-[{eta}-D-galactopyranosyl(1{ ightarrow}2)]-6-O-n-butyl;ester-{eta}-D-glucuronopyranoside$, respectively.