Tetracycline lyogel ointment consisting of hydroxy ethyl cellulose(HEC) in glycerin and Eudragit RS 100 in triacetin were prepared and then release characteristic were investigated. The physical properties of lyogel ointment such as viscosity, particle size and microscopic structures were also evaluated. The microscopic structures showed that lyogel particles containing drug were dispersed in the triactin solution. The release rate of drug from lyogel ointment as a function of HEC was not changed. However the release rate was significantly decresed when the amount of Eudragit RS 100 and triacetin in lyogel ointment was increased. The viscosity and weight fraction in external phase of lyogel ointment influenced the release rate. The current studies suggest that the release rate of drug can be controlled by changing of lyogel ointment compositions.