Effects of acupuncture at Sanyinjiao(SP6) and Yanglingquan(GB34) on bone mineral sensity(BMD) was investigated in ovariectomized(OVX) rats. BMD of OVX rats was measured left femur and tibia 2 and 4 weeks after acupuncture stimulation, respectively. Compared with sham-operated rats, a significant decrease in spine, pelvic and left femoral and tibial BMD was observed 2 and 4 weeks after OVX, respectively. In contrast, a significant increase of spinal, pelvic and left femoral and tibial BMD was elicited 2 and/or 4 weeks after acupuncture stimulation, respectively. These results show the possibility that acupuncture can play a role of ameliorating osteoporosis by elevating serum levels of female hormones related with bone metabolism.