In order to study the effect of Ontoyuklin-Tang (溫土毓麟湯), applied to cold of deficiency type sterility (虛)寒不姙) in women a series of expriments were conducted on the mouse regarding the in vitro developmental effect of I-cell embryos of the medium of Ontoyuklin- Tang, the ovulationin once a day of two-days, once a day for four-days, once every two-days irregualry for six-days after administered the drug orally, the change of weight, the in vitro developmental effect of 1-cell embryos and the level of serum of LH, FSH, Estradiol $17-{eta}$, Progesterone in mouse. The results were following. 1. The culture medium of the drug was not significantly increased in the number of zygotes and in vitro development of embryos. 2. The ovum of the mouse during ovulation, was increased by the administered drug, when treated once a day for two-days in comparison to once a day for four-days & once every two-days for six-days. 3. The level of weight of the mouse was not significantly increased after administering the drug in comparison to administering water. 4. The production of zygotes in the mouse was significantly increased after the drug was administered, but the effect of in vitro developmental effect on embros has a tendency to decrease. 5. The levels of serum LH and FSH in the mouse were not significantly increased after the drug administered, and the level of serum. Estradiol $17-{eta}$ of mice was few increased in a very small amaunt, but the level of the serum Progesterone of mice was significantly increased.