The aqua-acupuncture blended with liquid medicine and acupuncture is known to be effective in treating various medical disorders. This study used gallbladders of bear (WoongDam, WD, Ursus arctos Linne) and pig(JeoDam, JD, Sus scrofa domestica Brisson) plus the stones from the ox gallbladder(WooHwang, WH, Bos taurus var, domesticus Gmelin) to produce the liquid medicine. For the acupuncture, acupoint of ST -36 which is located one finger breadth from the anterior crest of the tibia was selected. There were a couple of purposes in this experiment. First, the relationship between aqua-acupuncture and gastric activity was scrutinized. Secondly, the possibility of pig gallbladder as an alternative organ to bear gallbladder for making liquid medicine was evaluated. Thirdly, different injection concentration ratio of liquid medicine was tried to figure out the optimal dosage for the aqua-acupuncture. The data of the present results indicated that the mixtures of WD + WH and JD + WH have a similar hypotonic effect on the stomach. These findings illustrated that aqua-acupuncture or hydroacupuncture showed inhibitory effect on the stomach in addition to its well-known prophylactic value. Also the possible alternative medical organ of pig gallbladder was checked out since two mixtures illustrated very similar trends in suppressing the gastric activity. The injection concentration ratio was not very different between two mixtures in terms of gastric motility except a couple of ratio. In conclusion, the aqua-acupuncture is a promising way to control the gastric activity for better medical purposes and the gallbladder of pig was assessed as a prospective one for making a liquid medicine.