The influences of the Reynolds number and free-stream turbulence intensity on the flow separation behavior around a circular were investigated experimentally. The range of the Reynolds number and turbulence intensity considered are 10,000 ~ 45,000 and 0.3 ~ 6.8%, respectively. Because of ineffectiveness of using time-mean value of hot-film sensor signals in determining the separation location around the cylinder, a new method using phase-difference of hot-film sensor signals with hot-wire being located in shedding vortex is suggested. The validity of the present method is confirmed by the comparison with flow visualization.