This paper presents an implementation method of Profibus interface software using FMS(Fieldbus Message Specification). The Profibus interface software is implemented on PC which is widely used as an industrial computer as well as a commercial embedded controller called IUC(Intelligent Universal Controller). In order to enable the Profibus interface software to handle many application tasks and communication services, two kinds of real-time/multi-tasking operating system, OS-9 and CTask, are utilized. We also develop an experimental model of Profibus-based automation system, and evaluate the performance of the Profibus network. Through experiments, the user layer level message latency is measured with respect to the change of message length, message generation interval and TRT(Target Rotation Time). The results of experiment are compared with those of a simulation model which comprises only the physical and data link layers of Profibus. The results of this study shows that the message latency in the user layer level occupies fairly a large part of the total message latency.