The purpose of this study is to consider what influences the Spanish folk costumes had on the ones of Latin America on Acculturation Phenomenon during Spanish reign over Latin America. As a method of doing this, the researcher compared and analyzed the style of dress such as color, material, design and accessories of Spanish folk costumes and the ones of Latin America, classifying them by male costumes and female ones respectively, by studying reference books, literature, and photographs related to the folk costumes and visiting this areas. The results of the study were summarized as follows; First, we can find out Spanish folk costumes were accepted to the folk costumes of Latin America just as it were. Second, Spainish folk costumes have been developed to be suitable for the climatic, geographical cultural characteristics of Latin America. Third, the folk costumes of Latin America become much simpler by the influence of Spainish culture. Fourth, as a result of cultural contact, mixture with Spainish culture, native folk costumes of Latin America had more various style. In conclusion, Spanish government over Latin America had given a cultural homogeneousness and colonial culture of Spain and traditional culture of Latin America have been mixed and created the present unique folk costumes of Latin America. Therefore good understanding and use of the sense of beauty of folk clothes of Latin America influenced by Spain culture will be helpful to enlarge creativeness of design of modern fashion.