- 흰나리 인편으로부터 PR-Proteinase의 유도 및 특성 규명
- ㆍ 저자명
- 오순자
- ㆍ 간행물명
- 韓國資源植物學會誌
- ㆍ 권/호정보
- 1998년|11권 2호|pp.195-201 (7 pages)
- ㆍ 발행정보
- 한국자원식물학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Some morphological character were surveyed and PR-proteinases were induced and characterized from Lilium formosanum Wallace endeimc to Cheju island . Its flower characters were similar to those of white trumpet lilies(Lilium longiflorum Thunb) although its flowering period was later than that of white trumpet lilies and it hadd a wide range of variation among individuals. Six PR-proteinases(II-2, III-1, III-2, IV-1, IV-2 and V) were induced from bulbs by 2.5mM salicylic acid and almost excreted into the intercellular spaces. These PR-proteinases were strongly activated by Ca 2+, , whereas they were strongly inhibited by Cu2+ Co2+ and Fe2+ . Three PR proteinases(II-2, IV-1 and IV-2) were strongly inhibited by 1, 10 -phenanthroline, indicating that these enzymes are metallo-proteinases. Three PR-proteinases(III-1, III-2 and V) had a high sensitivity to PMSF and required $eta$-mercaptoethanol for their activities. These results indicate that these proteinases are cysteine proteinases.