Five hundred and seventy-eight healthy subjects (351 men and 227 women) with a mean age of 44.8 years (45.2 for men, 44.3 for women) participated in this study. The serum concentrations of $alpha$-tocopherol, eta$-carotene, $alpha$-carotene, zeaxanthin + lutein, cryptoxanthin, lycopene and retinol of normal Koreans were measured, and their relation to gender, age, BMI, alcohol consumption, cigarette smoking, and menopausal status were evaluated. The concentrations of $alpha$-tocopherol, carotenoids and retinol in serum were measured simultaneously by reverse phase HPLC (multi-wavelength, gradient and computerized automatic system). Average serum levels of $alpha$-tocopherol, eta$-carotene and retinol were 10.1$pm$0.41mu$g/dl, 33.1$pm$1.24mu$</TEX>g/dl, and 82.0$pm$1.63mu$g/dl for men and 11.1$pm$0.74mu$</TEX>g/ml, 48.1$pm$1.60mu$g/dl and 64.5$pm$1.96mu$</TEX>g/dl for women, respectively, Serum concentrations of $alpha$-tocopheol eta$-carotene, $alpha$-carotene, cryptoxanthin and lycopene appeared to be higher in women than in men. The serum concentrations of zeaxanthin + lutein increased with the increase in age of men and those of $alpha$-carotene and eta$-carotene increased with the increase in age of women. For men, current smokers showed significantly lower serum concentrations of eta$-carotene and $alpha$-carotene than ex-smokers (p <0.05) . Current drinkers also showed significantly lower serum eta$-carotene and zeaxanthin + lutein concentrations than ex-drinkers. For women, current smokers showed significantly love. serum concentrations of zeaxanthin+lutein and cryptoxanthin than ex-smokes (p<0.05). Men with BMI$geq$24 showed significantly lower serum concentrations of eta$-carotene, $alpha$-carotene, lycopene and cryptoxanthin than men with BMI < 20. The mean concentrations of $alpha$-tocopherol, eta$-carotene, $alpha$-carotene and lycopene far postmenopausal women were higher than those for premenopausal women (p<0.05). In conclusion, there were obvious differences in serum $alpha$-tocopherol and carotenoids levels depending on gender, age, cigarette-smoking habits, alcohol consumption, BMI and menopausal status. further studies are required to establish the normal levels of these vitamins for children and the elderly and to elucidate their roles in different disease states.