The objective of this study is to manufacture the low temperature waterless container that is compact and low cost for a live fish transportation. Using the low temperature water container, it makes observations on the optimal conditions such as the amount of dissolved oxygen, total ammonia and nitrite in seawater for determining the survival rate of live fish in short and long-term transportation. Using a sole as a live fish, the temperatures of $0^{circ}C$, 3$^{circ}C$, 5$^{circ}C$, 7$^{circ}C$, 15$^{circ}C$ were controled for there effects. The results of this investigation show that as the seawater temperature increased, the amount of oxygen decreased and there was a low temperature shock below 3$^{circ}C$. It was observed that the fish was died with 30$mell/ell$of ammonia. The optimal temperature is about 5$^{circ}C$ for live fish transportation to maintain best survival rate.