This study was conducted to evaluate the growth and flowering responses of Eustoma grandiflorum cv. Azmasul, Azmahoon and Azmazo influenced by duration of storage at 1$0^{circ}C$ of seedlings raised in warm season. The plants after cold treatment were cultivated in a glasshouse under natural day length with the minimum temperature of 12$^{circ}C$. The stem thickness and number of leaves of Azmasul and Azmahoon were not affected by duration of cold treatment However, plant height, internode length fresh and. dry weights were significantly increased in the four-week treatment. In addition, four-week treatment increased flower weight and width. Bays to flowering of Azmasul and Azmahoon in four-week treatment were shortened by 20 days compared to the control or two-week treatment. Shoot growth and flower Qualify of Azmazo under the four-week treatment were promoted. Four-week treatment reduced the days to flowering of Azmazo by 31 and 36 days compared to the control and two-week treatment. The results of this study show that optimum duration for storage at l$0^{circ}C$ for seedlings of Ewtoma grandiflorum cv Azmasul. Azmahoon and Azmazo grown in warm season is four weeks.