The contribution of this paper is to provide the methods for the production automation of potato microtuber using the vision process in growth monitoring. The first method deals with computation for the growth density in the primary growth process. The second method addresses cognition process to identify the number and the volume of potato microtuber in secondary growth process. The third is to decide whether potato microtubers are infected by a virus or bacteria in growth process. The computation for the growth density in the primary growth process uses the method of Labeling. The second and third methods use template matching based on color patterns. With the developed method using vision process, this experiment is capable of discriminating weekly growth-rate in primary growth process, 85% cognition rate in secondary process and identifying whether there are infections. Therefore, we conclude that our experimental results are capable of growth monitoring for mass production of potato microtubers.