The fuel injection type, in the gasoline engines of atomization of fuel droplet and its distribution hae influenced directly on the decision of engine performance and harmful emission. In this paper, atomization characteristics of fuel spray is investigated with microscopic visualization system. Particle motion analysis system is used to measure the SMD from fuel spray of air-assisted injector by initial factors such as temperature of ambient air and air-assisted pressure. As air-assist pressure and ambientair temperature increase, the SMD is decreased, and its variation is more stable.