In order to investigate the droplet size distribution and Sauter Mean Diameter in a ultra high pressure diesel spray, fuel was injected with ultra high pressure into the environments of high pressure and room temperature by an Electronic Hydraulic Fuel Injection System. Droplet size was measured with the immersion liquid sampling technique. The immersion liquid was used a mixture of water-methycellulose solution and ethanol. The Sauter Mean Diameter decreased with increasing injection pressure, with a decrease environmental pressure (back pressure) and nozzle diameter. Increasing the injection pressure makes the fuel density distribution of the spray more homogeneous. An empirical correlation was developed among injection pressure, air density, nozzle diameter and the Sauter Mean Diameter of spray droplets.