The lipids from the seeds of Pinus koraiensis mostly composed of triacylglycerols (TGs), in which linoleic acid (46.2 mol%) and oleic acid (25.6 mol%) are present as main components in the fatty acid composition. Surprisingly, they also have unusual fatty acids with ${Delta}^5$-double bond systems such as ${Delta}^{5.9.12}-C_{18:3}$ (16.0 mol%), ${Delta}^{5.9}-C_{18:2}$ (2.3 mol%) and ${Delta}^{5.11.14}-C_{20:3}$ (0.8 mol%). Saturated fatty acids of palmitic, stearic and arachidic acid were present in less than 8.0 mol%. TG was resolved into 17 fractions by reverse-phase HPLC according to so-called partition number (PN) suggested by Plattner, in which TG molecules with ${Delta}^{5}$-NMDB acyl chains eluted later than did those with ${Delta}^{9}$-MDB acyl radicals. $Ag^+$-HPLC separated the TG into 14 fractions more clearly than did those with ${Delta}^{9}$-MDB acyl radicals. $Ag^+$-HPLC separated the TG into 14 fractions more clearly than did reverse-phase HPLC, and the complexity of ${Delta}^{5.9.12}-C_{18:3}$ moiety with silver ion impregnated in the column bed was in the level between ${Delta}^{9.12.15}-C_{18:3}$ ($C_{18:3{omega}3}$) and $C_{18:2{omega}6}$ (${Delta}^{9.12}-C_{18:2}$). In the $Ag^+$-HPLC, it was found that the molecular species having a given-numbered double bonds widely spreaded in the acyl chains eluted earlier than those concentrated in one acyl chain. The main molecular species are $(C_{18:2{omega}6})_2/{Delta}^{5.9.12}-C_{18:3}$ (14.8 mol%), $C_{18:1{omega}9}/C_{18:2{omega}6})_2$ (12.8 mol%) and $C_{18:1{omega}9}/C_{18:2{omega}6}/{Delta}^{5.9.12}-C_{18:3}$ (10.9 mol%).