This study was carried out to assess the relationship between jobstrain, psychosocial distress and homocysteine. The study design was cross-sectional, and included 152 industrial workers in middle sized city. A self-adminstered questionnaire measured general characteristic, job strain and psychosocial distress. Blood was drawn into EDTA tube, and total plasma homocysteine was measured by HPLC. Homocysteine in the high job demand group was sagnificantly higher than that in the low job demand. Cholesterol in the high job control group was significantly higher than that in the low job control. Homocysteine was significantly higher in the high job strain group than that in the other group. Folate and vitamin B12 showed a significantly negative correlation with homocysteine. Multiple regression analysis was used to determine whether the psychosocial distress and other variable contributed to explaining the homocysteine. Stress correlates with the homocysteine.