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Application of FITC-conjugated lectin probes for the recognition and differentiation of some Korean coastal red tide microalgae
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  • Application of FITC-conjugated lectin probes for the recognition and differentiation of some Korean coastal red tide microalgae
  • Application of FITC-conjugated lectin probes for the recognition and differentiation of some Korean coastal red tide microalgae
저자명
Cho. Eun Seob,Seo. Gwi Moon,Lee. Sam Geun,Kim. Hak Gyoon,Lee. Sang Jun,Rhodes. Lesley L.,Hong. Yong-Ki
간행물명
Journal of fisheries science and technology
권/호정보
1998년|1권 2호|pp.250-254 (5 pages)
발행정보
한국수산학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Harmful micro algae isolated from Korean coastal waters, were tested with FITC-conjugated lectins and observed by epifluorescent microscopy to distinguish each other. Strain-specific sugar composition at the cell surface was suggested by the affinity of lectins to different microalgae. The micro algae Cochlodinium polykrikoides (CP-1) and Gymnodinium $A_3;(GA_{3-1};1)$, are morphologically similar, but exhibited different binding activity with the lectins ECA, HPA and WGA. In Peridiniales, the micro alga Alexandrium tamarense (AT) bound HPA and WGA, but Scrippsiella trochoidea (ST-1) did not bind those lectins. Three species of Prorocentrum also exhibited different binding specificity with HPA, PHA and SBA. A non­toxic Korean isolate of Heterosigma akashiwo (HA-2) bound ConA, PEA and UEA. These results suggest that lectins are useful in discriminating morphologically similar species, as well as different species or strains within the same genus.