The aim of this study was to measure the regional micro-shear bond strength of dentin bonding agents to dentin, and to investigate the relationship between the micro-shear bond strength and two dentinal characteristics ; Vickers hardness and remaining dentin thickness. Twenty-four freshly extracted, noncarious human molars were selected for this study. The materials tested in this study consisted of two commercially available dentin bonding agents (MAC-BOND, ONE-STEP) and two restorative light-cured composite resins (AELITEFIL, Z100). The occlusal or side surface of tooth crown was sectioned to expose dentin, and the exposed surface was finally polished with # 600 sandpaper. Four groups of application methods were used combining the filling materials and the dentin bonding agents. The composite resin-attached tooth specimens were embeded in a cold cure acrylic resin, and were cut with a low speed diamond saw to the dimension of 1mm $ imes$ 1mm. Nine specimens were obtained from each tooth. The cut specimens were divided into three groups depending on the position of the dentin bonding surface. The micro-shear bond strength, remaining dentin thickness, and dentinal hardness were measured. Experimental results were then statistically analyzed with ANOVA. t-test, Scheffe test, and regression analysis. From this experiment, the following results were obtained : 1. In the case of occlusal surface bonding, the pooled micro-shear bond strength of ONST-AELIT group (16.62 MPa) was significantly higher than that of MACB-AELIT group (9.91 MPa) (p<0.05). However, there was no significant difference in the micro-shear bond strength depending on the dentin position (p>0.05). 2. In the case of side surface bonding of crown, the pooled micro-shear bond strength of four different bonding groups was not significantly different among each other (p>0.05). However, in three of the test groups (ONST-AELIT, MACB-Z100, ONST-Z100), the micro-shear bond strength to the lower 1/3(III) position was significantly lower than that to middle 1/3(II) position of surface (p<0.05). 3. In the ONST-AELIT bonding group, the pooled micro-shear bond strength to the occlusal surface was significantly lower than that to the side surface of crown (p<0.05). 4. There was no significant correlation between the micro-shear bond strength and dentin hardness / remaining dentin thickness (p>0.05).