The purpose of this study was to identify the effect of voluntary activity or midterm examination on self-esteem, health-promoting lifestyle, immunoglobulin in Nursing college student. Voluntary activity provides positive emotion(higher score of self-esteem) and midterm examination provides high level stress to students. Emotion and stress affect immune system. Seventy-five and seventy-eight subjects were assigned to voluntary activity and midterm examination group. In analyzing data, paired and unpaired t-tests and ANCOVA were used. The results were as following : 1.1 Self-esteem of after-voluntary activity was increased significantly than before-voluntary activity. 1.2 Health-promoting lifestyle of after-voluntary activity was not increased significantly than before- voluntary activity. 1.3 Immunoglobulin of after-voluntary activity was not increased significantly than before-voluntary activity. 2.1 Self-esteem of after-midterm examination was not decreased significantly than before-midterm examination. 2.2 Health-promoting lifestyle of after-midterm examination was decreased significantly .than before-midterm examination. 2.3 Immunoglobulin of after-midterm examination was not decreased significantly than before-midterm examination. 3.1 Self-esteem of voluntary activity group and midterm examination group was not different significantly. 3.2 Health-promoting lifestyle of voluntary activity group and midterm examination group was different significantly. 3.3 Immunoglobulin of voluntary activity group and midterm examination group was not different significantly.