Corydalis Tuber has been used in traditional medicine for an analgesic, antispasmodic and gastric ulcers. For the quality control on this drug, isolation and quantitative determination of berberine and coptisine from Corydalis ternata Nakai (Papaveraceae) has been conducted by using HPLC method. Berberine and coptisine in quarternary alkaloidal fraction from the crude drug were separated on silicagel column using a $CHCl_3:MeOH;(85:15)$ and $CHCl_3:MeOH:H_2O;(70:30:4)$ as an eluent, and the average contents were about 0.93 and 0.36%.