복령의 인공재배기술을 개선하고 복령추출물의 생리활성을 검토하고자 본 연구를 수행하였다. 종균의 종류 및 접종법에 따른 검토결과, 종목 양면접종법이 균핵형성정도, 균핵 갯수, 균핵 생산수율 및 개당 평균 중량에서 가장 좋았으며, 톱밥 양면접종법이 균핵형성 및 생성수율에서 가장 낮았다 복령의 품질과 생산성을 종합하여 판단할 때, 최적 종균 및 접종방법은 종목 양면접종 > 종목 절단면접종 > 균핵 양면접종 > 톱밥 양면접종의 순이었다. 복령균핵으로부터 분리한 생리활성물질인 조 복령당의 함량은 83%이었고, 농도가 증가함에 따라 상대점도가 상승하였으며, 염의 첨가 농도에는 큰 영향을 받지 않았다. 항산화 활성(EDA, POV, TBA)을 측정한 결과, TBA가의 경우에는 천연 항산화제인 비타민 E와 유사한 활성을 나타내었으나, POV와 EDA에 의한 항산화활성 측정 결과에서는 BHT나 비타민 C보다 낮은 활성을 나타내었다.
This study was carried out to improve an artificial culture techniques and antioxidative activity of the crude extract isolated from sclerotia of Poria cocos(Fr.) Wolf. In the test of different spawns and inoculation method, the sclerotia formation, number of sclerotia and production yield were excellent in the both sides inoculation method of log spawn, whereas the both side inoculation method of sawdust spawn was poor in sclerotia formation and yield. The optimal spawn and inoculation method for the quality and productivity of P. cocos was in the order of log spawn (both sides inoculation > log spawn(cutting section inoculation) > sclerotia (both sides inoculation) > sawdust spawn (both sides inoculation). The physiological activity substance, crude extract content of P. cocos NIAST 13007 was about 83%. As the concentration of crude extracts increased, the relative viscosity tended to be increased. However, as the concentration of sodium chloride increased, the relative viscosity did not affected. In antioxidative activities, electron donating ability (EDA) of P. cocos was about 10% of butylated hydroxytoluene (BHT). Thiobarbituric acid (TBA) value was similar to that of the vitamin C, however the peroxide value (POV) was lower than those of BHT and vitamin C.
