MIC test of 16 chemotherapeutic agents was performed on 24 isolates of Edwardsiella tarda collected from flounders. They revealed resistance against combinations of ampicillin, amoxicillin, erythromycin, flumequine, doxycycline(DOXY), nalidixic acid, novobiocin, oxolinic acid, oxytetracycline(OTC), thiamphenicol(TP) and sulfonamide. Two strains carried transferable R plasmid encoding Otc Kanamycin Tp and Otc chloramphenicol Doxy Tetracycline Tp, respectively. The R plasmids were not similar each other on the basis of their digestion pattern of restriction endonuclease, suggesting distribution of different transferable R plasmid among E. tarda from flounders.