Enoxacin[1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-1,8-naphthyridine-3-carboxylic acid, ENX] is a new quinolone antibacterial agent. The method is based on the highly colored charge-transfer complex formation of this drug as a $pi$-electron donor with 7,7,8,8-tetracyanoquinodimethane(TCNQ) or chloranil(CL) as $pi$-acceptors. The colored products were measured spectrophotometrically at 842 nm and 552 nm for TCNQ and CL, respectively. The different experimental conditions are optimized. The linearities for TCNQ and CL were $1.6{;}mu extrm{g}/mL~32{;}mu extrm{g}/mL$ and $6.4{;}mu extrm{g}/mL~160{;}mu extrm{g}/mL$, respectively and colors were produced in non-aqueous media. This report describes a simple and rapid method for the analysis of enoxacin.