In this research, biological treatability test was conduced using seawater flocculated tannery wastewater by fixed biofilm reactor. During one cycle, the removal efficiency of organic corbon obtained with fixed biofilm process for treating tannery wastewater was considerably greater than that with activated sludge process. As the hydraulic retention time increased form 0.5day to 4day, removal efficiency of organic carbon was increased from 72% to 87.3%. Attached biomass in media increased with influent organic loading up to 29g MLSS/L, that could reduce the specific organic loading rate. The continual measurement of attached biomass was possible for the operation of the biofilm reactor. Equal and low nitrication rates were observed in both suspended growth activated sludge process and fixed biofilm process, despite commercial nitrifier was seeded. Through the process of treating the tannery wastewater, EC50 values which is measured by the use of Ceriopdaphnia dubia, were decreased to the extent of 50% after treatment of seawater flocculation and of 83% after biological treatment, respectively, compared to those of the untreated wastewater.