A newly developed typhoid vaccine was tested for subcutaneous and intramuscular irritationin male Ner Zealand White rabbits. In subcutaneous and intramuscular irritation tests, there were no observed clinical signs, body weight changes and gross pathologic findings at doses of 1 mg/ml and 0.0125 mg/ml during experimental period. However, in positive control (0.75% acetic acid), we could find various lesions that had hemorrhage, necrosis and infiltration of inflammation cells in both subcutaneous and muscular tissues. From these results, we suggest that typhoid vaccine is not irritant in subcutaneous and muscular tissue of rabbits.