To develop a natural antimicrobial dyed fabric, water extracts were prepared from Impatiens balsamina leaf and stem. Wool and silk fabrics dyed with Impatiens balsamina extract were tested for antimicrobial activities aganist Staphylococcus aureus KCTC 1928 (St. aureus) and Trichopyton mentagrophytes (T. mentagrophytes) which are used in antimicrobial test of fabrics. The results were as follows Antimicrobial activity of wool and silk dyed with Impatiens balsamina extract increased with of dyeing and concentration of dye bath. The dyed silk fabric had higher antimicrobial activity than the dyed wool fabric. The fabrics dyed with leaf extract had higher antimicrobial activity than those dyed with stem extract.