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동결보존한 돼지정액의 융해조건이 정자의 생존율과 첨체변화에 미치는 효과
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  • 동결보존한 돼지정액의 융해조건이 정자의 생존율과 첨체변화에 미치는 효과
  • Effects of Thawing Conditions on the Viability and Acrosomal Morphology of Cryopreserved Boar Semen
저자명
정영호,서경덕,김광식,심금섭,이장희
간행물명
韓國受精卵移植學會誌
권/호정보
1999년|14권 2호|pp.131-137 (7 pages)
발행정보
한국수정란이식학회
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정기간행물|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

This experiment was carried out to investigate the effects of osmolarity of thawing diluents, seminal plasma added in thawing diluents on the sperm viability and the effects of thawing temperature, the temparature of the thawing diluents on the sperm viability and acrosomal morphology of boar spermatozoa by the straw method. The result obtained were summarized as follows: 1. The sperm viablilty after thawing of the frozen semen was shown greater in the high osmolarity(392~492mOsm) than low osmolarity(300mOsm) in thawing diluent. The added levels of seminal plasma in thawing diluent did not affect the viability of frozen-thawed boar semen. 2. In terms of thawing temperature, the sperm viability was shown higher in the frozen semen thawed at 5$0^{circ}C$ for one min. (p<0.01) than those thawed at 2$0^{circ}C$ or 37$^{circ}C$ for one min. The sperm viability was not significant at the diluent temparature of 2$0^{circ}C$or 37$^{circ}C$ after thawing: but the sperm viability was higher in thawing diluent at 2$0^{circ}C$ than in that at 37$^{circ}C$. However, the effects of thawing temperature and diluent solution on normal acrosomal rate were not significant. 3. Cleavage rates of oocytes fertilized with frozen semen were 46.4% and 43.3%, respectively, which were thawed at 5$0^{circ}C$ for one min. and then diluted in mBTS medium at 2$0^{circ}C$or 37$^{circ}C$. To sum up, the sperm viability was shown greater at the high of thawing diluents of frozen boar semen. In terms of thawing conditions, the sperm viability was shown greater, when semen was thawed at a high temperature for a short time and then diluted at the same temperature as that in the straw.