A thermotolerant yeast Saccharomyces cerevisiae OE-16 was isolated from traditional Meju was investigated on their physiological characteristics and ethanol fermentation ability. Saccharomyces cerevisiae OE-16 were able to grow up to 45$^{circ}C$ and 40% of glucose. Saccharomyces cerevisiae OE-16 was also resistant to 15% of KCl 1,200ppm of Pb2+, Hg2+ and 500ppm of potassium sorbate. From 20% glucose media Saccharomyces cerevisiae OE-16 produced 83.4g per liter of ethanol at 3$0^{circ}C$ and 9.5g per liter of ethanol at 4$0^{circ}C$ for 72 hours