this paper presents the results of experiments on subsooling degree characteristics which was analyzed in accordance with various test conditions. The test water was cooled below freezing point temperature, was measured. The results showed that using city water as the test water in pyrex tube indicated small standard deviation (SD) compared to using distilled water, and minimum subcooling degree was 6.9$^{circ}C$ and 6.2$^{circ}C$, respectively. In addition, a critical subcooling degree in a pyrex tube was larger than that in a stainless steel tube about 0.7$^{circ}C$ for the same test water. It was also observed that the standard deviation of data for the distilled water showed decreasing tendency aw the increasing with cooling velocity. Then metaldehyde showed a considerable effect on the subcooling degree of distilled water as the ice nucleating substance with increase of test number.